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Table 1 Catalytic parameters of the cellodextrin phosphorylases

From: In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum: implication for cellulose catabolism

  CdpA CdpB CdpC
K m k cat kcat/Km K m k cat kcat/Km K m k cat kcat/Km
G2
G3 Detected activitya: 15.2 Detected activitya: ≈ 0.4 5.4 ± 0.5 7650.8 ± 828.5 1410.4 ± 24.5
G4 11.9 ± 0.7 2340.1 ± 171.0 197.2 ± 7.3 4.7 ± 0.3 59.9 ± 5.4 12.8 ± 0.8 Detected activitya: ≈ 2
G5 5.6 ± 0.6 2866.3 ± 140.0 515.2 ± 33.0 Detected activitya:  ≈ 2 Detected activitya: ≈ 2
  1. Km values are given in mM, kcat values are given in min−1, and kcat/Km values are given in min−1 mM−1
  2. aIn these experiments, kcat or Km value could not be determined due to low activity of the enzyme. The detected activities are given in µM min−1 and were measured using substrate at 1 mM, enzyme concentration at 1 µM, and incubation at 37 °C up to 24 h as in the case of CdpB and G3. (−) means no activity was detected. The data show the means and standard deviations of three independent experiments