In vitro and in vivo exploration of the cellobiose and cellodextrin phosphorylases panel in Ruminiclostridium cellulolyticum: implication for cellulose catabolism

Table 1 Catalytic parameters of the cellodextrin phosphorylases

K_m values are given in mM, k_cat values are given in min⁻¹, and k_cat/K_m values are given in min⁻¹ mM⁻¹
^aIn these experiments, k_cat or K_m value could not be determined due to low activity of the enzyme. The detected activities are given in µM min⁻¹ and were measured using substrate at 1 mM, enzyme concentration at 1 µM, and incubation at 37 °C up to 24 h as in the case of CdpB and G3. (−) means no activity was detected. The data show the means and standard deviations of three independent experiments

ISSN: 2731-3654