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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Identification and manipulation of Neurospora crassa genes involved in sensitivity to furfural

Fig. 1

Effect of furfural on growth of N. crassa wild-type and mutant strains in a 96-well plate assay, as measured at 490 nm. a Wells of a 96-well plate were inoculated with 4 × 105 conidia of the wild-type strain in VgS media supplemented with different concentrations (15–75 mM) of furfural. b Delay in lag phase as expressed in the time required for detectable initiation of culture growth, compared to the control lacking furfural (15, 30 and 60 mM). c Inhibition in relative growth, as determined by O.D. of cultures exposed to furfural (15, 30 and 60 mM) relative to that of the control, 20 h after inoculation. Asterisks in b, c indicate a significant difference between the mutant strains and wild-type subjected to the same treatment (*P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t test). The wild-type control is shown in pink. The values represent the average of at least six biological replicates

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