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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Modular pathway engineering of key precursor supply pathways for lacto-N-neotetraose production in Bacillus subtilis

Fig. 3

Identifying positive-acting enzymes in the UDP-GlcNAc and UDP-Gal pathways. a Schematic of the cassette used to overexpress gene in B. subtilis. b Schematic of the cassette used to knockout gene in B. subtilis. c LNTII, LNnT titer of BY08 ~ BY12 and the control host BY06. d LNTII, LNnT titer of BY13–BY17 and the control host BY04. Triplicate experiments were done, and error bars represent the standard deviation. * and** mean p < 0.05 and p < 0.01, respectively

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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