Table 1 Strains and plasmids used in this study
Names | Characteristics | References |
|---|---|---|
Strains | ||
BS168comK | B. subtilis 168 derivate, PxylA-comK | This work |
BY01 | BS168comK derivate, ΔamyE::P43-lacY | This work |
BY02 | BY01 derivate, P43-lgtA, P43-lgtB | This work |
BY03 | BY01 derivate/pP43NMK-lgtA-lgtB | This work |
BY04 | BY01 derivate, P43-lgtB/pP43NMK-lgtA | This work |
BY05 | BY04 derivate, P43-lgtB | This work |
BY06 | BY05 derivate, P43-lgtB | This work |
BY07 | BY06 derivate, P43-lgtB | This work |
BY08 | BY06 derivate, P43-pgi | This work |
BY09 | BY06 derivate, P43-glmS | This work |
BY10 | BY06 derivate, P43-glmM | This work |
BY11 | BY06 derivate, P43-glmU | This work |
BY12 | BY06 derivate, ΔnagBAΔnagBB::lox72 | This work |
BY13 | BY04 derivate, P43-pgi | This work |
BY14 | BY04 derivate, P43-pgcA | This work |
BY15 | BY04 derivate, P43-gtaB | This work |
BY16 | BY04 derivate, ΔtuaD | This work |
BY17 | BY04 derivate, P43-galE | This work |
BY18 | BY06 derivate, P43-pgi, P43-gtaB | This work |
BY19 | BY18 derivate, P43-glmS | This work |
BY20 | BY19 derivate, P43-glmM | This work |
BY21 | BY20 derivate, ΔnagBAΔnagBB::lox72 | This work |
BY22 | BY18 derivate, P43-galE | This work |
BY23 | BY19 derivate, P43-galE | This work |
BY24 | BY20 derivate, P43-galE | This work |
BY25 | BY21 derivate, P43-galE | This work |
BY26 | BY22 derivate, ΔtuaD::lox72 | This work |
BY27 | BY23 derivate, ΔtuaD::lox72 | This work |
BY28 | BY24 derivate, ΔtuaD::lox72 | This work |
BY29 | BY25 derivate, ΔtuaD::lox72 | This work |
BY30 | BY26 derivate, P43-pgcA | This work |
BY31 | BY27 derivate, P43-pgcA | This work |
BY32 | BY28 derivate, P43-pgcA | This work |
BY33 | BY29 derivate, P43-pgcA | This work |
Plasmids | ||
pDG-Cre | Kmr, Ampr, temperature sensitive in B. subtilis | [15] |
p7Z6 | pMD18-T containing lox71-zeo-lox66 cassette | [15] |
p7S6 p7C6 | pMD18-T containing lox71-spc-lox66 cassette pMD18-T containing lox71-cm-lox66 cassette | [15] [15] |
p7Z6P43 | p7Z6 containing P43 promoter | [22] |
p7C6P43 | p7C6 containing P43 promoter | [22] |
p7S6P43 | p7S6 containing P43 promoter | [22] |
pP43-lgtA-lgtB | pP43NMK derivate with lgtA and lgtB cloned | This work |
pP43-lgtA | pP43NMK derivate with lgtA cloned | This work |