Simultaneous nutrition removal and high-efficiency biomass and lipid accumulation by microalgae using anaerobic digested effluent from cattle manure combined with municipal wastewater

Table 2 The orthogonal experimental results of nutrient composite in ADEC medium

Number	Glucose (g/L)	NaNO₃ (g/L)	K₂HPO₄·3H₂O (g/L)	MgSO₄·7H₂O (g/L)	Citric acid (g/L)	Trace elements (mL/L)	Biomass concentration (g/L)
1	10	0.3	0.010	0	0	0	4.08
2	6	0.4	0.010	0	0	0	2.42
3	10	0.2	0.020	0	0.006	0	3.74
4	6	0.2	0.010	0.075	0.006	1	2.44
5	8	0.2	0.010	0	0	1	3.52
6	8	0.3	0.010	0.075	0.006	0	3.36
7	6	0.4	0.010	0.075	0.006	0	2.38
8	6	0.2	0.010	0	0	1	2.64
9	10	0.2	0.010	0.075	0.006	1	4.04
10	8	0.4	0.020	0	0.006	1	2.80
11	6	0.3	0.015	0	0.006	1	2.44
12	8	0.2	0.015	0.075	0	0	3.26
13	6	0.2	0.020	0.075	0	0	2.48
14	6	0.2	0.015	0	0.006	0	2.48
15	10	0.4	0.015	0.075	0	1	4.14
16	6	0.3	0.020	0.075	0	1	2.48
Biomass concentration
K₁	2.438	3.244	3.291	3.174	3.286	3.184
K₂	3.203	3.259	3.261	3.231	3.119	3.221
K₃	3.968	3.104	3.056
R	1.530	0.155	0.235	0.057	0.168	0.037

“K_i” means average value of examination target at level i of each factor (i = 1, 2, 3). “R” means the range of K_i of each factor

ISSN: 2731-3654