Metabolic engineering of Bacillus amyloliquefaciens for enhanced production of S-adenosylmethionine by coupling of an engineered S-adenosylmethionine pathway and the tricarboxylic acid cycle

Table 1 Comparison of SAM production in this study with previous reports in methionine-free medium

Strain	Titer (mg/L)	Yield (mg/g DCW)	Productivity (mg/L h)	Medium carbon/nitrogen source (g/L)
B. amyloliquefaciens HSAM3	290.35	30.28	4.84	Sucrose 40, peptone 10, beef extract 5, urea 2, (NH₄)₂SO₄ 6.3, flask batch fermentation
B. amyloliquefaciens HSAM3	648.99	46.42	10.82	Sucrose 120, peptone 30, beef extract 15, urea 6, (NH₄)₂SO₄ 18.9, flask semi-continuous batch fermentation
E. coli Anti-argB [15, 18]	1.21	0.13	0.10	Glucose 20, NH₄Cl 0.5, flask batch fermentation
E. coli SSP-1 [13, 18]	5.30	Not available	0.44	Glucose 20, NH₄Cl 0.5, flask batch fermentation
C. glutamicum HW104/pJYW-4-metK-vgb [15]	196.7	12.15	4.10	Glucose 100, corn steep liquor 20, (NH₄)₂SO₄ 20, flask batch fermentation

ISSN: 2731-3654