Table 2 Strains and plasmids used in this study
Strains or plasmids | Characteristics | Source |
|---|---|---|
B. amyloliquefaciens strains | ||
 HZ-12 | CCTCC M2015234, wild type | Stored in lab |
 HZ-12 (PBmetK) | HZ-12 harboring the plasmid PBmetK | This study |
 HZ-12 (PSAM2) | HZ-12 harboring the plasmid PSAM2 | This study |
 HZ-12 (PBmetA) | HZ-12 harboring the plasmid PBmetA | This study |
 HZ-12 (PEmetA) | HZ-12 harboring the plasmid PEmetA | This study |
 HZ-12 (PBmetI) | HZ-12 harboring the plasmid PBmetI | This study |
 HZ-12 (PEmetB) | HZ-12 harboring the plasmid PEmetB | This study |
 HZ-12 (PYML) | HZ-12 harboring the plasmid PYML | This study |
 HZ-12ΔmccA | HZ-12 deficient in mccA | This study |
 HZ-12:SAM2 | HZ-12 integrated with SAM2 from S. cerevisiae | This study |
 HZ-12:SAM2(PthrA) | HZ-12:SAM2 harboring the plasmid PthrA | This study |
 HZ-12:SAM2(PmetL) | HZ-12:SAM2 harboring the plasmid PmetL | This study |
 HZ-12ΔmetEATAT | HZ-12 deficient in anti-antiterminator of metE | This study |
 HZ-12ΔsucC | HSAM1 deficient in sucC | This study |
 HSAM1 | HZ-12 integrated with SAM2, metA and metB, and deficient in mccA | This study |
 HSAM2 | HSAM1 deficient in sucC | This study |
 HSAM3 | HSAM2 harboring the plasmid PSAM2 | This study |
 E. coli DH5α | F− Φ80d/lacZΔM15, Δ(lacZYA-argF) U169, recA1, endA1, hsdR17 (r −K , m +K ), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Stored in lab |
 S. cerevisiae | CICC 31001, wild type | Stored in lab |
 B. subtilis 168 | Strain containing the P43 promoter | Stored in lab |
 B. licheniformis WX-02 | CCTCC M208065, wild type | Stored in lab |
Plasmids | ||
 pHY300PLK | E. coli–Bacillus shuttle vector for gene expression, Apr, Tetr | Stored in lab |
 PBmetK | pHY300PLK + P43 + TamyL + metK from HZ-12 | This study |
 PSAM2 | pHY300PLK + P43 + TamyL + SAM2 from S. cerevisiae | This study |
 PBmetA | pHY300PLK + P43 + TamyL + metA from HZ-12 | This study |
 PEmetA | pHY300PLK + P43 + TamyL + metA from E. coli DH5α | This study |
 PBmetI | pHY300PLK + P43 + TamyL + metI from HZ-12 | This study |
 PEmetB | pHY300PLK + P43 + TamyL + metB from E. coli DH5α | This study |
 PYML | pHY300PLK + P43 + TamyL + YML082 W from S. cerevisiae | This study |
 PthrA | pHY300PLK + P43 + TamyL + thrA from E. coli DH5α | This study |
 PmetL | pHY300PLK + P43 + TamyL + metL from E. coli DH5α | This study |
 T2(2)-ori | E. coli–Bacillus shuttle vector for gene knockout or integration; Kanr | Stored in lab |
 T2ΔmccA | T2 (2)-mccA (A + B) to delete mccA | This study |
 T2ΔmetEATAT | T2 (2)-metEATAT (A + B) to delete anti-antiterminator of metE | This study |
 T2ΔsucC | T2 (2)-sucC (A + B) to delete sucC | This study |
 T2-EmetA | T2 (2) + P43 + TamyL + metA from E. coli DH5α | This study |
 T2-EmetB | T2 (2) + P43 + TamyL + metA from E. coli DH5α | This study |
 T2-SAM2 | T2 (2) + P43 + TamyL + SAM2 from S. cerevisiae | This study |