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Table 2 Kinetic parameters for the oxidation of p-methoxybenzyl alcohol by AAO in the presence of different FFCA concentrations

From: Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase

[FFCA] (mM)

kcat (s−1)

Km (µM)

kcat/Km (s−1mM−1)

0

84.5 ± 2.1

35.3 ± 2.8

2390 ± 200

0.4

68.0 ± 3.3

33.0 ± 5.0

2120 ± 350

0.8

78.9 ± 1.7

37.9 ± 2.5

2080 ± 140

1.5

73.8 ± 3.4

37.0 ± 5.1

1990 ± 290

3.0

66.9 ± 2.3

31.8 ± 3.6

2100 ± 250

6.0

61.9 ± 2.6

27.6 ± 2.6

2250 ± 220

  1. Activity was followed by measuring the H2O2 released using AmplexRed® and HRP at 25 °C in air-saturated (0.256 mM O2) 50 mM sodium phosphate, pH 6.0