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Table 2 Kinetic parameters for the oxidation of p-methoxybenzyl alcohol by AAO in the presence of different FFCA concentrations

From: Complete oxidation of hydroxymethylfurfural to furandicarboxylic acid by aryl-alcohol oxidase

[FFCA] (mM) kcat (s−1) Km (µM) kcat/Km (s−1mM−1)
0 84.5 ± 2.1 35.3 ± 2.8 2390 ± 200
0.4 68.0 ± 3.3 33.0 ± 5.0 2120 ± 350
0.8 78.9 ± 1.7 37.9 ± 2.5 2080 ± 140
1.5 73.8 ± 3.4 37.0 ± 5.1 1990 ± 290
3.0 66.9 ± 2.3 31.8 ± 3.6 2100 ± 250
6.0 61.9 ± 2.6 27.6 ± 2.6 2250 ± 220
  1. Activity was followed by measuring the H2O2 released using AmplexRed® and HRP at 25 °C in air-saturated (0.256 mM O2) 50 mM sodium phosphate, pH 6.0