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Table 1 Comparison of sugar consumption and product formation in batch fermentation of strains SCUT27 and SCUT27/∆nfnAB with different carbon sources

From: Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Strain Carbon source Sugar uptake (mmol/L) Lactic acid concentration (mmol/L) Acetic acid concentration (mmol/L) Ethanol concentration (mmol/L) Hydrogen concentration (mmol/L) Hydrogen yield (mol/mol) Biomass (mmol/L) Carbon recovery (%) Electron recovery (%)
SCUT27 Glucose 29.17 ± 2.06 25.44 ± 1.89 15.17 ± 1.33 28.48 ± 2.61 34.92 ± 3.04 1.20 ± 0.09 37.67 ± 2.79 91.59 ± 4.92 96.71 ± 6.98
Xylose 32.73 ± 2.2 20.78 ± 1.56 13.67 ± 0.67 26.52 ± 1.96 31.14 ± 2.13 0.95 ± 0.06 32.56 ± 2.33 94.67 ± 7.29 93.69 ± 7.16
G:X = 2:1 30.77 ± 1.95 24.33 ± 1.33 14.67 ± 0.83 27.39 ± 1.52 33.15 ± 1.96 1.12 ± 0.07 33.95 ± 3.26 92.18 ± 6.81 93.59 ± 7.62
SCUT27/∆nfnAB Glucose 28.94 ± 1.78 22 ± 1.67** 14.67 ± 1.01 32.17 ± 2.39** 49.28 ± 2.58** 1.70 ± 0.12** 36.74 ± 2.79 93.72 ± 5.49 98.21 ± 6.75
Xylose 32.53 ± 1.73 18.78 ± 1.44* 13.5 ± 0.83 28.7 ± 2.39* 44.79 ± 3.78** 1.37 ± 0.08** 32.09 ± 2.33 93.67 ± 6.19 92.38 ± 5.88
G:X = 2:1 30.14 ± 2.36 20.78 ± 1.56** 14.5 ± 0.67 31.3 ± 1.96* 45.97 ± 2.86** 1.63 ± 0.13** 35.35 ± 2.79 94.75 ± 7.16 96.91 ± 6.25
  1. Asterisks indicate significant differences between SCUT27 and SCUT27/∆nfnAB (**p ≤ 0.01; *p ≤ 0.05; t test). The carbon in biomass was estimated using the general empirical formula for cell composition of CH2N0.25O0.5 [22]. mol/mol means mol H2/mol consumed sugar (glucose and/or xylose)