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Table 1 Comparison of sugar consumption and product formation in batch fermentation of strains SCUT27 and SCUT27/∆nfnAB with different carbon sources

From: Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Strain

Carbon source

Sugar uptake (mmol/L)

Lactic acid concentration (mmol/L)

Acetic acid concentration (mmol/L)

Ethanol concentration (mmol/L)

Hydrogen concentration (mmol/L)

Hydrogen yield (mol/mol)

Biomass (mmol/L)

Carbon recovery (%)

Electron recovery (%)

SCUT27

Glucose

29.17 ± 2.06

25.44 ± 1.89

15.17 ± 1.33

28.48 ± 2.61

34.92 ± 3.04

1.20 ± 0.09

37.67 ± 2.79

91.59 ± 4.92

96.71 ± 6.98

Xylose

32.73 ± 2.2

20.78 ± 1.56

13.67 ± 0.67

26.52 ± 1.96

31.14 ± 2.13

0.95 ± 0.06

32.56 ± 2.33

94.67 ± 7.29

93.69 ± 7.16

G:X = 2:1

30.77 ± 1.95

24.33 ± 1.33

14.67 ± 0.83

27.39 ± 1.52

33.15 ± 1.96

1.12 ± 0.07

33.95 ± 3.26

92.18 ± 6.81

93.59 ± 7.62

SCUT27/∆nfnAB

Glucose

28.94 ± 1.78

22 ± 1.67**

14.67 ± 1.01

32.17 ± 2.39**

49.28 ± 2.58**

1.70 ± 0.12**

36.74 ± 2.79

93.72 ± 5.49

98.21 ± 6.75

Xylose

32.53 ± 1.73

18.78 ± 1.44*

13.5 ± 0.83

28.7 ± 2.39*

44.79 ± 3.78**

1.37 ± 0.08**

32.09 ± 2.33

93.67 ± 6.19

92.38 ± 5.88

G:X = 2:1

30.14 ± 2.36

20.78 ± 1.56**

14.5 ± 0.67

31.3 ± 1.96*

45.97 ± 2.86**

1.63 ± 0.13**

35.35 ± 2.79

94.75 ± 7.16

96.91 ± 6.25

  1. Asterisks indicate significant differences between SCUT27 and SCUT27/∆nfnAB (**p ≤ 0.01; *p ≤ 0.05; t test). The carbon in biomass was estimated using the general empirical formula for cell composition of CH2N0.25O0.5 [22]. mol/mol means mol H2/mol consumed sugar (glucose and/or xylose)