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Table 2 Comparison of sugar consumption and product formation by strains SCUT27 and SCUT27/∆nfnAB using dilute acid pretreated substrate in serum bottles with 20 mM/L CaCO3

From: Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Strain Dilute acid pretreated substrate Sugar uptake (mmol/L) Hydrogen concentration (mmol/L) Hydrogen productivity (mmol/L h) Hydrogen yield (mol/mol) Lactic acid production (mmol/L) Acetic acid production (mmol/L) Ethanol production (mmol/L)
SCUT27 Rice straw 37.45 ± 1.82 43.38 ± 2.63 3.61 ± 0.22 1.16 ± 0.06 30.44 ± 1.56 15.17 ± 1.17 35 ± 2.39
Corn cob 41.29 ± 2.25 49.47 ± 2.57 4.12 ± 0.21 1.21 ± 0.05 31.89 ± 2 20.33 ± 2.16 38.48 ± 2.83
Corn straw 44.56 ± 2.11 39.79 ± 2.11 2.65 ± 0.14 0.91 ± 0.06 33.11 ± 1.78 17.33 ± 1.83 40.65 ± 2.39
Wheat straw 47.72 ± 2.56 38.35 ± 2.23 3.20 ± 0.19 0.81 ± 0.05 35.11 ± 2 19 ± 2.17 45.43 ± 2.39
Soybean straw 39.02 ± 1.95 33.69 ± 2.03 2.81 ± 0.17 0.84 ± 0.07 28 ± 1.33 15 ± 1.67 38.48 ± 2.61
Sorghum straw 40.82 ± 2.37 39.59 ± 1.87 3.63 ± 0.16 0.99 ± 0.04 31 ± 1.56 14 ± 2.5 38.91 ± 2.61
SCUT27/∆nfnAB Rice straw 41.51 ± 2.13* 62.39 ± 3.45** 5.20 ± 0.29** 1.48 ± 0.11** 28.67 ± 1.78* 15.33 ± 1.5 43.26 ± 2.61**
Corn cob 42.33 ± 2.59 69.76 ± 2.78** 5.81 ± 0.23** 1.65 ± 0.09** 27.67 ± 1.22** 20.33 ± 1.5 44.78 ± 3.26**
Corn straw 43.98 ± 1.84 54.33 ± 2.15** 3.62 ± 0.14** 1.25 ± 0.07** 29.44 ± 1.56** 17.83 ± 1.67 47.39 ± 2.61**
Wheat straw 48.35 ± 2.67 54.26 ± 2.83** 4.52 ± 0.24** 1.16 ± 0.05** 31 ± 1.67** 18.67 ± 2.67 51.96 ± 2.39**
Soybean straw 42.38 ± 2.23* 46.35 ± 2.85** 3.86 ± 0.24** 1.09 ± 0.07** 26.11 ± 2.11* 14.83 ± 1.33 41.52 ± 3.04*
Sorghum straw 41.69 ± 2.51 53.37 ± 2.97** 4.45 ± 0.25** 1.28 ± 0.09** 26.89 ± 1.89** 13.83 ± 1.67 45.65 ± 3.04**
  1. Asterisks indicate significant differences between SCUT27 and SCUT27/∆nfnAB (**p ≤ 0.01; *p ≤ 0.05; t test). Biomass was not measured due to the addition of CaCO3. mol/mol means mol H2/mol consumed sugar (mainly glucose and xylose)