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Table 2 Comparison of sugar consumption and product formation by strains SCUT27 and SCUT27/∆nfnAB using dilute acid pretreated substrate in serum bottles with 20 mM/L CaCO3

From: Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

Strain

Dilute acid pretreated substrate

Sugar uptake (mmol/L)

Hydrogen concentration (mmol/L)

Hydrogen productivity (mmol/L h)

Hydrogen yield (mol/mol)

Lactic acid production (mmol/L)

Acetic acid production (mmol/L)

Ethanol production (mmol/L)

SCUT27

Rice straw

37.45 ± 1.82

43.38 ± 2.63

3.61 ± 0.22

1.16 ± 0.06

30.44 ± 1.56

15.17 ± 1.17

35 ± 2.39

Corn cob

41.29 ± 2.25

49.47 ± 2.57

4.12 ± 0.21

1.21 ± 0.05

31.89 ± 2

20.33 ± 2.16

38.48 ± 2.83

Corn straw

44.56 ± 2.11

39.79 ± 2.11

2.65 ± 0.14

0.91 ± 0.06

33.11 ± 1.78

17.33 ± 1.83

40.65 ± 2.39

Wheat straw

47.72 ± 2.56

38.35 ± 2.23

3.20 ± 0.19

0.81 ± 0.05

35.11 ± 2

19 ± 2.17

45.43 ± 2.39

Soybean straw

39.02 ± 1.95

33.69 ± 2.03

2.81 ± 0.17

0.84 ± 0.07

28 ± 1.33

15 ± 1.67

38.48 ± 2.61

Sorghum straw

40.82 ± 2.37

39.59 ± 1.87

3.63 ± 0.16

0.99 ± 0.04

31 ± 1.56

14 ± 2.5

38.91 ± 2.61

SCUT27/∆nfnAB

Rice straw

41.51 ± 2.13*

62.39 ± 3.45**

5.20 ± 0.29**

1.48 ± 0.11**

28.67 ± 1.78*

15.33 ± 1.5

43.26 ± 2.61**

Corn cob

42.33 ± 2.59

69.76 ± 2.78**

5.81 ± 0.23**

1.65 ± 0.09**

27.67 ± 1.22**

20.33 ± 1.5

44.78 ± 3.26**

Corn straw

43.98 ± 1.84

54.33 ± 2.15**

3.62 ± 0.14**

1.25 ± 0.07**

29.44 ± 1.56**

17.83 ± 1.67

47.39 ± 2.61**

Wheat straw

48.35 ± 2.67

54.26 ± 2.83**

4.52 ± 0.24**

1.16 ± 0.05**

31 ± 1.67**

18.67 ± 2.67

51.96 ± 2.39**

Soybean straw

42.38 ± 2.23*

46.35 ± 2.85**

3.86 ± 0.24**

1.09 ± 0.07**

26.11 ± 2.11*

14.83 ± 1.33

41.52 ± 3.04*

Sorghum straw

41.69 ± 2.51

53.37 ± 2.97**

4.45 ± 0.25**

1.28 ± 0.09**

26.89 ± 1.89**

13.83 ± 1.67

45.65 ± 3.04**

  1. Asterisks indicate significant differences between SCUT27 and SCUT27/∆nfnAB (**p ≤ 0.01; *p ≤ 0.05; t test). Biomass was not measured due to the addition of CaCO3. mol/mol means mol H2/mol consumed sugar (mainly glucose and xylose)