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Table 3 Strains, plasmids and primers used in this study

From: Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates

 

Description

Reference or source

Strain

 T. aotearoense SCUT27

Wild-type

Our lab

 T. aotearoense SCUT27/∆nfnAB

nfnAB deletion mutant of SCUT27

This study

 E. coli DH5α

Used for plasmid screening and propagation

TianGen

Plasmid

 pBluescript II SK+

Standard cloning vector

Stratagene

 pPuKAd

As the template for kanamycin resistance gene amplification

[44]

 pBlunAB

Disrupts nfnAB with kanamycin resistance gene

This study

Primer

Sequence (5′–3′)

 

nfnA-F

GGTACCCCTTGCAGGCATTTCTTCC

 

nfnA-R

GAATTCGAAGGTTGCCCTGTTCACG

 

nfnB-F

GGATCCCCAACTGTGACTCTGCATC

 

nfnB-R

GAGCTCAGAAGAAGCAATTGAATCC

 

aph-F

GGATCCGATAAACCCAGCGAACC

 

aph-R

GAATTCATCGATACAAATTCCTCGTAGG

 

M13-F

CGCCAGGGTTTTCCCAGTCACGAC

 

M13-R

AGCGGATAACAATTTCACACAGGA

 

probe-F

TTTGCTCGGAAGAGTATGAA

 

probe-R

GCCACTTACTTTGCCATCT