Description | Reference or source | |
---|---|---|
Strain | ||
T. aotearoense SCUT27 | Wild-type | Our lab |
T. aotearoense SCUT27/∆nfnAB | nfnAB deletion mutant of SCUT27 | This study |
E. coli DH5α | Used for plasmid screening and propagation | TianGen |
Plasmid | ||
pBluescript II SK+ | Standard cloning vector | Stratagene |
pPuKAd | As the template for kanamycin resistance gene amplification | [44] |
pBlunAB | Disrupts nfnAB with kanamycin resistance gene | This study |
Primer | Sequence (5′–3′) | |
---|---|---|
nfnA-F | GGTACCCCTTGCAGGCATTTCTTCC | |
nfnA-R | GAATTCGAAGGTTGCCCTGTTCACG | |
nfnB-F | GGATCCCCAACTGTGACTCTGCATC | |
nfnB-R | GAGCTCAGAAGAAGCAATTGAATCC | |
aph-F | GGATCCGATAAACCCAGCGAACC | |
aph-R | GAATTCATCGATACAAATTCCTCGTAGG | |
M13-F | CGCCAGGGTTTTCCCAGTCACGAC | |
M13-R | AGCGGATAACAATTTCACACAGGA | |
probe-F | TTTGCTCGGAAGAGTATGAA | |
probe-R | GCCACTTACTTTGCCATCT |