Table 1 Yeast strains used in this study

Y58

Xylose-utilizing strain, H145E10-XYLA3-1, derived from H131-A3^CS (BF264-15Dau background), and then evolved

MATa, leu2-3, 112, URA3, trp1-1∆::(P_TDH3-RKI1-T_CYC1, P_TDH3-RPE1-T_CYC1, TRP1), his2∆::(P_TDH3-TKL1-T_CYC1, HIS2), ade1∆::(P_TDH3-PsTAL1-T_CYC1, ADE1), ChVI::(P_TDH3-PsXYL3-T_CYC1, 32 copies of P_TDH3-PsXYLA-T_CYC1), arg4∆::(GRE3^E193K, ARG4)

[19, 26]

YZy165

Y58, δ-IbOH pathway

Y58 (δ-integration-ILV2, ILV5, ILV3, CoxIV_MLSARO10, CoxIV_MLSLlAdhA^RE1)

This study

YZy171

δ-IbOH pathway, bat1Δ, ald6Δ, pho13Δ, ura3Δ

YZy181 ura3-K134stop

This study

YZy173

δ-IbOH pathway, bat1Δ

YZy165 bat1Δ::hphMX

This study

YZy176

bat1Δ, ald6Δ, pho13Δ, ura3Δ

Y58 bat1Δ::hphMX, ald6Δ::kanMX, pho13Δ::natMX, uar3-K134stop.

This study

YZy177

δ-IbOH pathway, bat1Δ, pho13Δ

YZy165 bat1Δ::hphMX, pho13Δ::natMX

This study

YZy178

δ-IbOH pathway, pho13Δ

YZy165 pho13Δ::natMX

This study

YZy181

δ-IbOH pathway, bat1Δ, ald6Δ, pho13Δ

YZy165 bat1Δ::hphMX, ald6Δ::kanMX, pho13Δ::natMX

This study

YZy182

δ-IbOH pathway, ald6Δ, pho13Δ

YZy165 ald6Δ::kanMX, pho13Δ::natMX

This study

YZy183

δ-IbOH pathway, ald6Δ

YZy165 ald6Δ::kanMX

This study

YZy184

δ-IbOH pathway, bat1Δ, ald6Δ

YZy165 bat1Δ::hphMX, ald6Δ::kanMX

This study

YZy197

YZy171, pJA180

YZy171 (2μ-ILVs + CoxIV_MLSLlKivd + CoxIV_MLSLlAdhA^RE1)

This study

YZy199

YZy171 (2µ_URA3_vector)

YZy171, pRS426

This study