Table 2 Plasmids used in this study

pRS426

Amp^R, 2μ, URA3

[65]

pJA180

(2μ-ILVs + CoxIV_MLS-LlKivd + CoxIV_MLS-LlAdhA^RE1)

Amp^R, 2μ, URA3, P_TDH3-ScILV2-HA-T_ADH1-P_PGK1-ScILV3-His-T_CYC1-P_TEF1-CoxIV_MLS-LlAdhA^RE1-cMycTag-T_ACT1-[P_TDH3-CoxIV_MLS-LlKivd-cHATag- T_ADH1-P_TEF1-ScILV5-cMycTag-T_ACT1]

[2]

pJA182

(2μ-ILVs + CoxIV_MLS-ARO10 + CoxIV_MLS-LlAdhA^RE1)

Amp^R, 2μ, URA3, P_TDH3-ScILV2-HA-T_ADH1-P_PGK1-ScILV3-His-T_CYC1-P_TEF1-CoxIV_MLS-LlAdhA^RE1-cMycTag-T_ACT1-[P_TDH3-CoxIV_MLS-ScARO10-cHATag-T_ADH1-P_TEF1-ScILV5-cMycTag-T_ACT1]

[2]

pYZ17

Amp^R, Lox71-kanMX-Lox66 gene-disruption cassette

This study

pYZ23

Amp^R, Lox71-bleMX6-Lox66 δ-integration vector

[41]

pYZ34

(δ-integration-ILVs, CoxIV_MLS-ARO10, CoxIV_MLS-LlAdhA^RE1)

Amp^R, δ-integration-Lox71-ShBle-Lox66-P_TDH3-ScILV2-HA-T_ADH1-P_PGK1-ScILV3-His-T_CYC1-P_TEF1-CoxIV_MLS-LlAdhA^RE1-cMycTag-T_ACT1-[P_TDH3-CoxIV_MLS-ScARO10-cHATag-T_ADH1-P_TEF1-ScILV5-cMycTag-T_ACT1]

This study

pYZ55

Amp^R, Lox71-hphMX-Lox66 gene-disruption cassette

This study

pYZ84

Amp^R, Lox71-natMX-Lox66 gene-disruption cassette

This study