Skip to main content

Table 2 Plasmids used in this study

From: Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol in Saccharomyces cerevisiae

Plasmid Description (brackets indicate reverse direction) Source
pRS426 AmpR, 2μ, URA3 [65]
pJA180 (2μ-ILVs + CoxIVMLS-LlKivd + CoxIVMLS-LlAdhARE1)
AmpR, 2μ, URA3, PTDH3-ScILV2-HA-TADH1-PPGK1-ScILV3-His-TCYC1-PTEF1-CoxIVMLS-LlAdhARE1-cMycTag-TACT1-[PTDH3-CoxIVMLS-LlKivd-cHATag- TADH1-PTEF1-ScILV5-cMycTag-TACT1]
[2]
pJA182 (2μ-ILVs + CoxIVMLS-ARO10 + CoxIVMLS-LlAdhARE1)
AmpR, 2μ, URA3, PTDH3-ScILV2-HA-TADH1-PPGK1-ScILV3-His-TCYC1-PTEF1-CoxIVMLS-LlAdhARE1-cMycTag-TACT1-[PTDH3-CoxIVMLS-ScARO10-cHATag-TADH1-PTEF1-ScILV5-cMycTag-TACT1]
[2]
pYZ17 AmpR, Lox71-kanMX-Lox66 gene-disruption cassette This study
pYZ23 AmpR, Lox71-bleMX6-Lox66 δ-integration vector [41]
pYZ34 (δ-integration-ILVs, CoxIVMLS-ARO10, CoxIVMLS-LlAdhARE1)
AmpR, δ-integration-Lox71-ShBle-Lox66-PTDH3-ScILV2-HA-TADH1-PPGK1-ScILV3-His-TCYC1-PTEF1-CoxIVMLS-LlAdhARE1-cMycTag-TACT1-[PTDH3-CoxIVMLS-ScARO10-cHATag-TADH1-PTEF1-ScILV5-cMycTag-TACT1]
This study
pYZ55 AmpR, Lox71-hphMX-Lox66 gene-disruption cassette This study
pYZ84 AmpR, Lox71-natMX-Lox66 gene-disruption cassette This study