Table 3 A list of primers used in this study
Primers | Sequences (5′➝3′) |
|---|---|
Pyruvate-to-lactyl-CoA module | |
DL_0001 | CATCATCACCACCATCACTAA |
DL_0002 | ATGTATATCTCCTTCTTATAGTTAAAC |
DL_0032 | TAGAAATAATTTTGTTTAACTATAAGAAGGAGATATACATATGAAACTCGCCGTTTATAG |
DL_0033 | GGGAACCTTTCTCATTATATCTCCTTTTAAACCAGTTCGTTCGGGC |
DL_0034 | ACGAACTGGTTTAAAAGGAGATATAATGAGAAAGGTTCCCATTAT |
DL_0035 | GCCGCTCTATTAGTGATGGTGGTGATGATGTCAGGACTTCATTTCCTTCAG |
Pyruvate-to-lactate ester module | |
DL_0013 | GAGCCTCAGACTCCAGCGTA |
DL_0014 | ATATCAAGCTTGAATTCGTTACCCGG |
DL_0015 | GGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATTAATACGACTCACTATAGGG |
DL_0016 | GTCCAGTTACGCTGGAGTCTGAGGCTC |
Upstream module | |
JW_0001 | GGGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCATGAAACTCGCCGTTTATAGC |
JW_0002 | CTAAATAGGTACCGACAGTATAACTCATTATATCTCCTTTTAAACCAGTTCGTTCGGGC |
JW_0003 | CGAAACCTGCCCGAACGAACTGGTTTAAAAGGAGATATAATGAGTTATACTGTCGGTACC |
JW_0004 | CGCAAGCTTGTCGACCTGCAGGCGCGCCGAGCTCGAATTCTTAGAAAGCGCTCAGGAAG |
JW_0005 | GGATCCTGGCTGTGGTGATGA |
JW_0006 | GAATTCGAGCTCGGCGCG |
Downstream module | |
JW_0007 | GTATATTAGTTAAGTATAAGAAGGAGATATACATATGATGAGAAAGGTTCCCATTATTAC |
JW_0008 | GAAATTATACTGACCTCAATTTTCTCCATTATATCTCCTTTCAGGACTTCATTTCCTTC |
JW_0009 | AATGGGTCTGAAGGAAATGAAGTCCTGAAAGGAGATATAATGGAGAAAATTGAGGTCAG |
JW_0010 | CAAATTTCGCAGCAGCGGTTTCTTTACCAGACTCGAGTCAATATCTTGAAATTAGCGTCT |
JW_0011 | CATATGTATATCTCCTTCTTATACTTAACT |
JW_0012 | CTCGAGTCTGGTAAAGAAAC |
Synthetic operons for upstream module | |
JW_0013 | GGGAATTGTGAGCGGATAACAATTCCCCAAGGAGATATAATGAAACTCGCCGTTTATAGC |
JW_0014 | TTATGCTAGTTATTGCTCAGCGGTGGCGGCCGCTCTATTATTAAACCAGTTCGTTCGG |
JW_0015 | TCTGGAAAAAGGCGAAACCTGCCCGAACGAACTGGTTTAATAATAGAGCGGCCGC |
JW_0016 | GATTATGCGGCCGTGTACAATACGATTACTTTCTGTTCGATTTCTACCGAAGAAAGGC |
JW_0017 | CATTATATCTCCTTGGGGAATTGTTATCCGC |
JW_0018 | TCGAACAGAAAGTAATCGTATTG |
JW_0019 | AAATTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCATGAGTTATACTGTCGGTACC |
JW_0020 | GCGTTCAAATTTCGCAGCAGCGGTTTCTTTACCAGACTCGAGTTAGAAAGCGCTCAGGAA |
JW_0021 | AAATCTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCATGAGTTATACTGTCGGTACC |
JW_0022 | CATGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAAATTTCGATTATGCGGCC |
JW_0023 | CATGCTAGCATTATACCTAGGACTGAGCTAGCTGTCAGATTTCGATTATGCGGCC |
JW_0024 | TACAGTGCTAGCAGCTTAGCGACAACCCTAGGCGCTCGCATGAGTTATACTGTCGGTACC |
JW_0025 | GTATAATGCTAGCTTAGCAGTACCAGGACGTACCGGAGTATGAGTTATACTGTCGGTACC |
JW_0026 | TAGGTACAGTGCTAGCACTAGGCCTAGCGATTCCGCTAAATGAGTTATACTGTCGGTACC |
JW_0027 | TATAATGCTAGCAGTTTACCTAGGGCAATAGCGTACCGAATGAGTTATACTGTCGGTACC |
JW_0028 | CATGCGAGCGCCTAGGGTTGTCGCTAAGCTGCTAGCACTGTACCTAGG |
JW_0029 | CATTTAGCGGAATCGCTAGGCCTAGTGCTAGCACTGTACCTAGG |
JW_0030 | CATACTCCGGTACGTCCTGGTACTGCTAAGCTAGCATTATACCTAGG |
JW_0031 | CATTCGGTACGCTATTGCCCTAGGTAAACTGCTAGCATTATACCTAGG |
Synthetic operons for downstream module | |
JW_0032 | TTATGCTAGTTATTGCTCAGCGGTGGCGGCCGCTCTATTATCAGGACTTCATTTCCTTCA |
JW_0033 | TGCAGAAGGCTTAATGGGTCTGAAGGAAATGAAGTCCTGATAATAGAGCGGCCGC |
JW_0034 | GATTATGCGGCCGTGTACAATACGATTACTTTCTGTTCGATTTCTACCGAAGAAAGGC |
JW_0035 | GATATAGCTCGAACGCGGAAAGAGATGAGAAAGGTTCCCATTATTAC |
JW_0036 | TCAGGACTTCATTTCCTTCA |
JW_0037 | GCAACCTATTTTAATCCAAGGAAGATCTAATGAGAAAGGTTCCCATTATTAC |
JW_0038 | GCAATAACAACTAGGAGAGACGACATGAGAAAGGTTCCCATTATTAC |
JW_0039 | TAATGGGAACCTTTCTCATCTCTTTCCGCGTTCGAGCTATATCGGGGAATTGTTATCCGC |
JW_0040 | TGCAGAAGGCTTAATGG |
JW_0041 | GGAACCTTTCTCATTAGATCTTCCTTGGATTAAAATAGGTTGCGGGGAATTGTTATCCGC |
JW_0042 | TAATGGGAACCTTTCTCATGTCGTCTCTCCTAGTTGTTATTGCGGGGAATTGTTATCCGC |
JW_0043 | TAACCAAAACACTAACGCAAGATGGAGAAAATTGAGGTCAGT |
JW_0044 | AGGGCACGAGGAGGAACCAGTAGAATGGAGAAAATTGAGGTCAGT |
JW_0045 | GCAACCAACACAACGAGGAGGCATTTAATGGAGAAAATTGAGGTCAGT |
JW_0046 | TACTGACCTCAATTTTCTCCATCTTGCGTTAGTGTTTTGGTTAGGGGAATTGTTATCCGC |
JW_0047 | CTCAATTTTCTCCATTCTACTGGTTCCTCCTCGTGCCCTGGGGAATTGTTATCCGC |
JW_0048 | CTCAATTTTCTCCATTAAATGCCTCCTCGTTGTGTTGGTTGCGGGGAATTGTTATCCGC |