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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes

Fig. 2

PvC3H72 subcellular localization and transcriptional activity assay. a Subcellular localization of PvC3H72–GFP fusion protein in Arabidopsis protoplast. GFP protein alone shows fluorescent signals in nucleus, membrane, and cytoplasm. DAPI is a staining dye for the nucleus. Bars = 5 µm. b PvC3H72 transcriptional activity in yeast system. GUS was used as a negative control. c Schematic diagram of three plasmids used for transactivation activity. Three plasmids (effector, reporter and internal control) were electroporated into Arabidopsis protoplasts at the ratio of 5:4:1. d PvC3H72 showed transactivation activity in the plant cells. All the experiments were repeated three times with similar results. Letters above bars indicate significant difference at P < 0.05 (n = 3)

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