Fig. 4

Confirmation of PvC3H72-overexpression (OE-72) transgenic lines. a Schematic diagram of T-DNA used for genetic transformation. b Southern blot for WT and two OE-72 transgenic lines. The binary vector used for transformation was the positive control (PCK). c Relative expression of PvC3H72 in WT and two OE-72 lines using qRT-PCR. Letters above bars indicate significant difference at P < 0.05 (n = 3)