Fig. 2
From: Discovery and implementation of a novel pathway for n-butanol production via 2-oxoglutarate
Butanol titer (mg L−1) for the different strain designs in TB medium and HDM medium. The different enzymes expressed in each strain are indicated. In all experiments, strains were grown in shake flasks until reaching 0.4–0.5 OD600. 0.5 mM of IPTG was then added to the medium and cells transferred to sealed serum bottles. Data are shown as mean ± SD from three independent experiments. ND not detected; the detection limit of the method is 3 mg L−1. 2OG 2-oxoglutarate, 2-HdxG 2-hydroxyglutarate, HdxG-CoA 2-hydroxyglutaryl-CoA, Gtc-CoA glutaconyl-CoA, Crt-CoA crotonyl-CoA, But-CoA butanoyl-CoA, ButOH butanol, acCoA ACETYL-CoA, AcAcCoA acetoacetyl-CoA, 3-HB-CoA 3-hydroxybutyryl-CoA, Cit citrate, Lact lactate, OAA oxaloacetate, PEP phosphoenolpyruvate