Fig. 2
Isolation of singlet-oxygen-resistant (SOR) lines of C. vulgaris. a SOR-1, SOR-5 and SOR-6 strains were isolated as a 1O2-resistant mutant by growing on solid TAP medium either containing (+) or not (−) of Red Bengal (RB 12 μM). The amount of cells spotted is indicated in the left border. b Quantitative analyses of the resistance of SOR strains to oxidative stress. Cells were grown in liquid cultures with increasing concentration of RB (0–50 µM) for 24 h, then were spotted on TAP-agar for recovery. Plates were illuminated with 100 μmol photons m−2 s−1, photoperiod of 16-/8-h light/dark, 25 °C