Fig. 3

Schematic of gene editing using CRISPR-Cas9 in B. napus. a Single-gRNA (upper) and multi-gRNA (lower) mediated gene editing in B. napus. E, editing; N-E, non-editing. b Single-gRNA mediated target for knockout at CRISPR-Cas9-mediated cleavage. Sequencing results in chrA07 are listed in g1 lines, g2 lines and g3 lines, respectively, and in the common site of g4 lines. c Multi-gRNA-mediated target for knockout at CRISPR-Cas9-mediated cleavage. “−” and “+” indicate nucleotide insertions and deletions in the target sequence, while “s” indicates substituted mutations, and “c” indicates combined mutations. The pentagram in red indicates insertions in the target site. gRNAs are coloured yellow, whereas PAMs are coloured green