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Table 1 Transformation efficiencies of different gRNAs in CRISPR-Cas9-mediated transgenic lines

From: Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

  gRNA-sc Mutant Total Transgenic efficiency (%) On-target efficiency (%)
g1 21 6 25 84 29
g2 39 16 48 81 41
g3 40 11 41 98 28
g4 87 59 93 97 68
g123 30 5 30 100 17
g134 10 3 10 100 30
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