Table 1 Transformation efficiencies of different gRNAs in CRISPR-Cas9-mediated transgenic lines
| Â | gRNA-sc | Mutant | Total | Transgenic efficiency (%) | On-target efficiency (%) |
|---|---|---|---|---|---|
g1 | 21 | 6 | 25 | 84 | 29 |
g2 | 39 | 16 | 48 | 81 | 41 |
g3 | 40 | 11 | 41 | 98 | 28 |
g4 | 87 | 59 | 93 | 97 | 68 |
g123 | 30 | 5 | 30 | 100 | 17 |
g134 | 10 | 3 | 10 | 100 | 30 |