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Table 2 Potential off-target activity for each sgRNA target site in transgenic B. napus

From: Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

  1. Nucleotides in the green background are consistent with the designed sgRNA target sites. Mismatched nucleotides are in lower case. The PAM is highlighted with a red background
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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