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Table 2 Plasmids used in this study

From: Efficient production of d-lactate from methane in a lactate-tolerant strain of Methylomonas sp. DH-1 generated by adaptive laboratory evolution

Plasmid

Description

References

pCM184

AmpR, KanR, TetR; broad-host range allelic exchange vector

[34]

pDel-glgA-Lm.LDH

pCM184-UglgA-[Lm.LDH-TrrnB-KanR]-DglgA

This study

pDel-glgA-Lj1.LDH

pCM184-UglgA-[Lj1.LDH-TrrnB-KanR]-DglgA

This study

pDel-glgA-Lj3.LDH

pCM184-UglgA-[Lj3.LDH-TrrnB-KanR]-DglgA

This study

pDel-glgA-Ld.LDH

pCM184-UglgA-[Ld.LDH-TrrnB-KanR]-DglgA

This study

pDel2-glgA-Lm.LDH

pDel-glgA-Lm.LDH without AmpR

This study

pIns

Plasmid containing [UIns-TrrnB-KanR-DIns] cassette for integration into noncoding region between AYM39_05845 and AYM39_05850

This study

pIns-mW

pIns-[PwatR (ΔTT)-watR-TrrnB]

This study

pIns-mSR

pIns-[PwatR (ΔTT)- smtM, rstM-TrrnB]

This study

pIns-mWSR

pIns-[PwatR (ΔTT)-watR, smtM, rstM-TrrnB]

This study

pDel2-fliE

pDel2-UfliE-[TrrnB-KanR]-DfliE

This study

pDel2-SR

pDel2-UsmtM-[TrrnB-KanR]-DrstM

This study

pDel2-WSR

pDel2-UwatR-[TrrnB-KanR]-DrstM

This study