Fig. 4

Transcript levels of xyr1 in the overexpression strain TX(WT). The wild-type-like strain Δtmus53, the Xyr1-deficient strain Xyr1′(81), and the Xyr1-overexpression strain TX(WT) were cultivated on the indicated carbon sources and samples were taken after 24 h (glucose, glycerol, xylan, lactose) or 48 h (CMC). Relative transcript levels of xyr1 were measured by RT-qPCR analysis, normalized to the reference sample (Δtmus53, glycerol, indicated by asterisk) using the reference genes sar1 and act1. Mean values are given; error bars indicate standard deviation from three independently grown cultures