Fig. 7

Transcript levels of the major cellulase-encoding genes. The wild-type-like strain Δtmus53, the Xyr1-deficient strain Xyr1′(81), the Xyr1-overexpression strain TX(WT), and the fusion TF bearing strain TXY(1) were cultivated on the indicated carbon sources and samples were taken after 24 h (glucose, glycerol, xylan, lactose) or 48 h (CMC). Relative transcript levels of the indicated genes were measured by RT-qPCR analysis, normalized to the reference sample (Δtmus53, glycerol, indicated by asterisk) using the reference genes sar1 and act1. Mean values are given; error bars indicate standard deviation from three independently grown cultures