Skip to main content

Table 2 Primers used in this study

From: Fusion transcription factors for strong, constitutive expression of cellulases and xylanases in Trichoderma reesei

Name

Sequence (5′–3′)

5Xyr1_fwd

TGTACATGTATGATGGCGTGC

Xyr1*_250rev-BamHI

CAGTACCCGTTGAATGGATCCTCTACCTGGCAGCAATAAGAGAGC

Xyr1*_250fwd-BamHI

CTTATTGCTGCCAGGTAGAGGATCCATTCAACGGGTACTGCTGGG

TXyr1_rev-KpnI

GGTACCATCAAGCCCTCTTCACTTTCAGC

Xyr1_3fwd-KpnI

GTACCGGGCTTGATTCACAGAATGATTC

Xyr1_3rev-NotI

GCGGCCGCTTCTTCTACTTCAAAGCTTTGGCAG

Ppki_5fwd

AGATAACGGTGAGACTAGCGGC

Tcbh2_rev_BcuI

ACTAGTGCTATTAACGTTTGGAAAGCCATC

Ptef_fwd-BspEI

TCCGGAGAGTTGGGCAAAATCAGGC

Ptef_rev-MCS

ACTAGTCTACGCTAGCGACCCATATGGATCTTAAGTGTGATGTAGCGTGAGAGCTG

Xyr1-fwd-NdeI

CATATGTTGTCCAATCCTCTCCGTC

Xyr1-rev-NheI

GCTAGCTAGAGGGCCAGACCGGTTC

Xyr1_P336r-MCS

GCTAGCGACCTCCAATTGCTCCGACGTCGCCATTAATGGGCTGCGAGAGCTG

Ypr1_L185f-MunI

CAATTGTTCTTACTCCACAGTCGACAACG

102499_rev-NheI

GCTAGCCGTAAATGCTCCCATC

Ypr2_P204f-MunI

CAATTGTTCCCCGGAATGTTGTGCTC

Ypr2_rev-SpeI

ACTAGTCTAGTAGTTCGTTCTCCTTCCAGTG

5Xyr1_fwd2

CGGAAGATACGATGGAGGAAC

Xyr1wt_Test_250rev

GCAGTACCCGTTGAATTCTTC

Xyr1*_Test_250rev

TACCCGTTGAATGGATCCTCTAC

5pyr4_fwd3

CCAGACGGTGATTCACATATACG

Ptef_rev-BspTI

CTTAAGTGTGATGTAGCGTGAGAGCTG

pyr4_3fwd

AGACGAGGACCAGCAGACC

Tpyr4_rev2

CAGGAAGCTCAGCGTCGAG

Xyr1_1760rev-NotI

GCGGCCGCGTTCAAGTCGTGCTCATCCAC

5pyr4_fwd(BglII)

GCGGAAGATCTCGAGATAGTATCTC

5pyr4_rev-BspEI

TCCGGAGTAGCTCTTCACTGGTTGTGGTG

sar1fw

TGGATCGTCAACTGGTTCTACGA

sar1rev

GCATGTGTAGCAACGTGGTCTTT

act1f

TGAGAGCGGTGGTATCCACG

act1r

GGTACCACCAGACATGACAATGTTG

xyr1_q2f

TCCGTCGCTATTCTGCCTAC

xyr1_q2r_wt_1

CAGCAGTACCCGTTGAATTC

cbh1f

GATGATGACTACGCCAACATGCTG

cbh1r

ACGGCACCGGGTGTGG

cbh2f

CTATGCCGGACAGTTTGTGGTG

cbh2r

GTCAGGCTCAATAACCAGGAGG

egl1f

CTGCAACGAGATGGATATCCTGG

egl1r

GTAGTAGCTTTTGTAGCCGCTGC

bgl1_q1f

ATCATTCTGGAGCAGATTCTTG

bgl1_q1r

GTAAGACAGTCCATAGCCGAAC

xyn1f

CAGCTATTCGCCTTCCAACAC

xyn1r

CAAAGTTGATGGGAGCAGAAG

xyn2_q1f

CCGTCAACTGGTCCAACTCG

xyn2_q1r

GTGCGGTAAATGTCGTAGACG

bxl1_q1f

GAATGACATGAACCTCCGACC

bxl1_g1r

CGAAGGTGAAGACGGGAATC

xyl1-fwd

CTGTGACTATGGCAACGAAAAGGAG

xyl1-rev

CACAGCTTGGACACGATGAAGAG