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Table 2 Primers used in this study

From: Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger

Primer name

F/R

Sequence 5′–3′

Template

332 AclA-3,600 F

F

TCAGCCCAGGTCT-TGTTCAG

A. carbonarius acl2 ORF

333 AclA-4,237 R

R

CCCTCCATGTCCA-ATGATAAGGA

A. carbonarius tef1 promoter

334 AclB-3,910 F

F

TCCCTTCCCTCGCT-TCTCTC

A. nidulans tef1 promoter

335 AclB-4,497 R

R

ATGGCCTTGCTGA-CATCCTG

A. carbonarius acl1 ORF