Skip to main content

Table 3 Transcriptome data of known cytosolic acetyl-CoA generating pathways and the genes related to heterologous IA production in A. niger

From: Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in Aspergillus niger

Locus tag

Enzyme

Old locus tag

Localization

AB1.13 WT

AB1.13 CAD

RPKM

RPKM

 

ATP-citrate lyase

      

ANI_1_76094

ATP-citrate lyase subunit 1 (aclA)

An11g00510

Cyto

325.61

325.45

506.32

505.54

ANI_1_78094

ATP-citrate lyase subunit 2 (aclB)

An11g00530

Cyto

331.2

332.23

587.4

587.02

 

CAT system

      

ANI_1_724074

Carnitine acetyl transferase (facC)

An08g04990

Cyto/Nucleus

28.78

29.17

24.89

24.77

ANI-1-192164

Carnitine acetyl transferase (acuJ)

An18g01590

Per/Mito

45.97

47.7

38.2

38.08

ANI_1_388034

Carnitine/acyl carnitine carrier (acuH)

An03g03360

Cyto/Mito

31.08

30.93

29.01

28.95

 

PAA pathway

      

ANI_1_936024

Pyruvate decarboxylase (pdcA)

An02g06820

Cyto

5910.75

5915.54

304.97

306.49

ANI_1_1024084

Pyruvate decarboxylase (pdcB)

An09g01030

Cyto

38.82

38.64

47.95

48.26

ANI_1_2276014

Pyruvate decarboxylase

An01g01590

Nucleus

0.35

0.45

0.17

0.18

ANI_1_796114

Pyruvate decarboxylase

An13g03320

Mito/Cyto

0

0

0.03

0.03

ANI_1_1024074

Aldehyde dehydrogenase (aldA) 

An08g07290

Cyto

143.48

142.19

178.07

177.72

ANI_1_226174

Aldehyde dehydrogenase

An10g00850 

Per

1.59

1.35

1.69

2.21

ANI_1_1748184

Aldehyde dehydrogenase

An04g03400

Cyto

54.25

54.85

53.22

54.15

ANI_1_924184

Acetyl-CoA synthetase (acuA)

An04g05620

Cyto

139.46

141.21

96.66

96.09

ANI_1_938144

Acetyl-CoA hydrolase (ach1)

An16g07110

Mito

98.53

99.38

22.36

22.93

ANI_1_878024

Acetate kinase

An02g06420

Cyto/Mito

42.53

43.41

13.72

13.75

 

Mitochondrial acetyl-CoA synthesis

      

ANI_1_1206064

Pyruvate dehydrogenase E1 component subunit alpha (pda1)

An07g09530

Mito

263.81

264.72

205.40

207.09

ANI_1_622094

Pyruvate dehydrogenase E1 component subunit alpha

An11g04550

Mito

10.81

9.47

10.54

9.75

ANI_1_12014

Pyruvate dehydrogenase E1 component subunit beta

An01g00100

Mito

215.18

213.19

177.40

176.43

ANI_1_274064

Pyruvate dehydrogenase E2 component

An07g02180

Mito

299.81

301.04

286.65

284.54

 

Citrate synthases

      

ANI_1_876084

Citrate synthase (citA)

An09g06680

Mito

478.40

482.85

428.47

426.67

ANI_1_1226134

Methylcitrate synthase (mcsA)

An15g01920

Mito

51.49

51.55

25.69

24.11

ANI_1_1474074

Citrate synthase (citB)

An08g10920

Cyto

58.09

57.90

522.44

521.45

ANI_1_2950014

Citrate synthase (citC)

An01g09940

Cyto

3.86

3.52

0.91

0.96

 

Aconitases

      

ANI_1_1410074

Aconitate hydratase (aco1)

An08g10530

Mito

234.09

234.83

397.94

397.68

ANI_1_470084

Aconitate hydratase

An09g03870

Mito

64.88

64.41

26.01

26.07

ANI_1_3018024

Aconitate hydratase

An02g11040

Cyto

0.04

0.04

0.00

0.00

ANI_1_1808144

Aconitate hydratase

An16g05760

Cyto

0.85

0.85

0.98

0.99

ANI_1_578044

Aconitase

An05g02230

Cyto

6.29

6.38

14.13

14.28

ANI_1_1802134

Aconitase

An15g07730

Cyto

26.63

26.21

30.62

30.72

 

Itaconate biosynthesis

      
 

cis-aconitate decarboxylase (cadA)

 

Cyto

1.27

1.20

4287.14

4300.13

  1. Gene expression data as obtained from Hossain et al. [17] are given in RPKM (Reads assigned Per Kilobase of target per Million mapped reads) values and calculated according to the method presented by Mortazavi et al. [41]
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us