Fig. 2

Dependence of the [H₂O₂]_t=0 and \(v_{{\left( {{\text{H}}_{2} {\text{O}}_{2} } \right)}}\) on pre-incubation time of LF at 25 °C. Reactions were made in 50 mM sodium acetate (pH 5.0) at 25 °C. The concentration of CNWs was 1.0 g L⁻¹ and that of LF was 10% (v/v). a Time curves of the formation of soluble products (expressed in N-acetylglucosamine equivalents, NAG_eq) upon incubation of CNWs with SmLPMO10A in the presence of LF that was pre-incubated at 25 °C for different times (as indicated in the plot). The graphs shows average values, and S.D, obtained from experiments with SmLPMO10A concentrations of 0.05 µM, 0.1 µM, or 0.25 µM (see Additional file 1: Fig. S2 for the individual curves). Solid lines show linear regression of the data according to Eq. 2. b, c Dependence of (b) the [H₂O₂]_t=0 and (c) the \(v_{{\left( {{\text{H}}_{2} {\text{O}}_{2} } \right)}}\), on pre-incubation time of LF. The values of [H₂O₂]_t=0 and \(v_{{\left( {{\text{H}}_{2} {\text{O}}_{2} } \right)}}\) were calculated from the parameter values of linear regression analysis of the data in a according to Eq. 2 using the stoichiometry (n) of 4 NAG_eq/H₂O₂ for the SmLPMO10A/CNWs system [48]. Error bars represent S.D. and are from three independent measurements each made in single parallel but at different concentration of SmLPMO10A (Additional file 1: Fig. S2)