Fig. 3

Liquid fraction (LF) supports the degradation of cellulose (BMCC) by TrLPMO9A. Reactions were made in 50 mM sodium acetate (pH 5.0) at 50 °C. The concentration of BMCC was 0.6 g L⁻¹ (except in one series where it was 1.2 g L⁻¹, red diamonds). Before use in the LPMO reaction, the LF was pre-incubated at 50 °C for 0.5 h. All colored points and lines refer to reactions with LF with a concentration 10%, except for the yellow series where it was 20% (v/v). In summary: green squares, base case; red diamonds, double BMCC concentration; yellow circles, double LF. The blue triangles are from an experiment where the concentration of LF was 10% but the reactions were supplied with 20-µM H₂O₂ at t = 0. The green arrow shows the increase in intercept upon supplementation of the reaction with 20-µM H₂O₂. The graphs shows the average values, and S.D, obtained from experiments with TrLPMO9A concentrations (each in single parallel) of 0.1 µM, 0.2 µM, or 0.5 µM (see Additional file 1: Fig. S5 for the individual curves). The data points show the formation of soluble products (expressed in glucose equivalents, Glc_eq). Solid lines show linear regression of the data according to Eq. 2. Control experiments show the results obtained (i) with 10% LF but in the presence of 1 µM HRP (black circles), (ii) with 10% LF but in the absence of TrLPMO9A (black diamonds), or (iii) in the absence of LF (black crosses)