Fig. 4

Stoichiometry of the TrLPMO9A reaction at 50 °C. Reactions were made in 50 mM sodium acetate (pH 5.0) at 50 °C. The concentrations of BMCC, LF and TrLPMO9A were 0.6 g L⁻¹, 10% (v/v) and 0.5 µM, respectively. Before use in the LPMO reaction, the LF was pre-incubated at 50 °C for 24 h. All reactions were supplemented with H₂O₂ (10–50 µM, final concentrations) just before starting the reaction by addition of TrLPMO9A. Error bars represent S.D. and are from at least two independent measurements. a Time curves for the formation of soluble products (expressed in glucose equivalents, Glc_eq) upon incubation of BMCC with TrLPMO9A in the presence of externally added H₂O₂. Solid lines show linear regression of the data according to Eq. 2. b Dependency of intercept values obtained from linear regression of the data shown in panel A on the concentration of added H₂O₂. The solid line shows linear regression of the data and the slope of the line suggests stoichiometry of 1.32 ± 0.11 Glc_eq/H₂O₂ for the TrLPMO9A/BMCC system