Time course of d-limonene and l-limonene production in the engineered Y. lipolytica strains. The cells of Po1g KdLS and Po1g KlLS were cultivated at 30 °C and 200 rpm with an initial OD600 of 0.1 in 30 mL of liquid YPD medium in a 250-mL shake flask, and limonene titers were determined at the 2-, 3-, 4-, 5-, 7- and 9-day time-points. To avoid loss of limonene during cultivation, 8% of n-dodecane overlay was added into the YPD medium prior to cultivation.
: production of d-limonene by Po1g KdLS.
: production of l-limonene by Po1g KlLS. Bars represent limonene titers. All values presented are the mean of three biological replicates ± standard deviation