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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Engineering the oleaginous yeast Yarrowia lipolytica to produce limonene from waste cooking oil

Fig. 5

Effect of volume of n-dodecane, initial OD600 and MgSO4·7H2O on d-limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR. a Effect of volume of n-dodecane on d-limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR. The cells were cultivated at the optimum fermentation temperature of 20 °C, the optimum rotation speed of 250 rpm, with an initial OD600 of 0.1 in 30 mL of liquid YPD medium in a 250-mL shake flask for 5 days with different volumes of n-dodecane (6%, 8%, 10%, and 12%). b Effect of initial OD600 on d-limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR. The cells were cultivated at the optimum fermentation temperature of 20 °C, the optimum rotation speed of 250 rpm, with an initial OD600 of 0.1 and 8% of n-dodecane in 30 mL of liquid YPD medium in a 250-mL shake flask for 5 days with different initial OD600 (0.01, 0.1, 1.0 and 2.0). c Effect of volume of MgSO4·7H2O on d-limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR. The cultivation was performed at the optimum fermentation temperature of 20 °C, the optimum rotation speed of 250 rpm, the optimum initial OD600 of 2.0, the optimum pH of 5.74 and the best n-dodecane volume 10% in the optimal 50 mL-final-volume of medium in a 250-mL shake flask for 5 days with different concentrations of MgSO4·7H2O (0, 0.1%, 0.2%, and 0.3%). : d-limonene accumulation of Po1g KdHR; : l-limonene accumulation of Po1g KlHR; : biomass accumulation of Po1g KdHR; : biomass accumulation of Po1g KlHR. All values presented are the mean of three biological replicates ± standard deviation

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