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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Engineering the oleaginous yeast Yarrowia lipolytica to produce limonene from waste cooking oil

Fig. 7

Effect of WCO on d-limonene or l-limonene accumulation in Po1g KdHR or Po1g KlHR. a Effect of WCO on d-limonene accumulation of Po1g KdHR. : d-limonene accumulation of Po1g KdHR from WCO; : d-limonene accumulation of Po1g KdHR from vegetable oil; : biomass accumulation of Po1g KdHR from WCO; : biomass accumulation of Po1g KdHR from vegetable oil. b Effect of WCO on l-limonene accumulation of Po1g KlHR. : l-limonene accumulation of Po1g KlHR from WCO; : l-limonene accumulation of Po1g KlHR from vegetable oil; : biomass accumulation of Po1g KlHR from WCO; : biomass accumulation of Po1g KlHR from vegetable oil. The cultivation of Po1g KdHR or Po1g KlHR was performed at the optimum fermentation temperature of 20 °C, the optimum rotation speed of 250 rpm, the optimum initial OD600 of 2.0, the optimum pH of 5.74, the best n-dodecane volume 10% and the optimum Mg2+ concentration 0.2% of MgSO4·7H2O in the optimal 50 mL-final-volume of WCO medium in a 250-mL shake flask for 5 days with different concentrations of WCO (0%, 10%, 30%, 50%, and 70%) as carbon source instead of glucose. Commercial vegetable oil was also used as a control. : d-limonene accumulation of Po1g KdHR from WCO; : l-limonene accumulation of Po1g KlHR from vegetable oil; : biomass accumulation of Po1g KdHR; : biomass accumulation of Po1g KlHR. All values presented are the mean of three biological replicates ± standard deviation

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