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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures

Fig. 2

Discovery of CATSa F97W responsible for enhanced activity towards isobutanol. a A binding pocket of CATSa and associated amino acid residues. The catalytic residues are in purple. b Protein sequence alignment of CATSa with different CATs. c Screening of F97 variants for enhanced isobutyl acetate production in E. coli. The letters indicate amino acids substituting F in the wild-type CATSa. d Superposed binding pocket structure of the wild-type and CATSa F97W mutant. The red arrow indicates a CH–π interaction between the hydrogen of isobutanol and the indole ring of F97W

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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