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Table 1 Kinetic parameters of the wild-type CATSa and mutant CATSa F97W

From: Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures

Substrates CATSa CATSa F97W
Chloramphenicol Isobutanol Chloramphenicol Isobutanol
KM (mM) 0.28 ± 0.02 138.66 ± 28.92 0.18 ± 0.01 144.77 ± 23.65
kcat (1/s) 202.97 ± 3.36 0.30 ± 0.03 102.63 ± 2.04 0.59 ± 0.05
kcat/KM (1/M/s) 7.37 ± 0.48 × 105 2.16 ± 0.45 5.77 ± 0.49 × 105 4.08 ± 0.62
  1. The reactions were performed at 50 °C. The co-substrate, acetyl-CoA, was supplied at the saturated concentration of 2 mM. Melting temperature (Tm) of CATSa and CATSa F97W is 72.0 ± 0.8 and 68.3 ± 1.2 °C, respectively