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Table 1 Kinetic parameters of the wild-type CATSa and mutant CATSa F97W

From: Single mutation at a highly conserved region of chloramphenicol acetyltransferase enables isobutyl acetate production directly from cellulose by Clostridium thermocellum at elevated temperatures

Substrates

CATSa

CATSa F97W

Chloramphenicol

Isobutanol

Chloramphenicol

Isobutanol

KM (mM)

0.28 ± 0.02

138.66 ± 28.92

0.18 ± 0.01

144.77 ± 23.65

kcat (1/s)

202.97 ± 3.36

0.30 ± 0.03

102.63 ± 2.04

0.59 ± 0.05

kcat/KM (1/M/s)

7.37 ± 0.48 × 105

2.16 ± 0.45

5.77 ± 0.49 × 105

4.08 ± 0.62

  1. The reactions were performed at 50 °C. The co-substrate, acetyl-CoA, was supplied at the saturated concentration of 2 mM. Melting temperature (Tm) of CATSa and CATSa F97W is 72.0 ± 0.8 and 68.3 ± 1.2 °C, respectively