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Table 1 Genetically encoded biosensors developed and applied to sense aromatic monomers and sugars towards lignin valorization

From: Genetically encoded biosensors for lignocellulose valorization

Target molecule(s) Sensing element Output element Host organism References
Aromatic monomers
 Protocatechuic acid PcaU Engineered PpcaU, P3B5B Escherichia coli [69, 101]
PcaU Engineered PpcaU Pseudomonas putida [70]
PobR variant Ppob Escherichia coli [75]
PcaV Engineered PPV Escherichia coli [76]
 Vanillin EmrR PemrR Escherichia coli [68]
Yap1/Msn2 Padh7 Escherichia coli [89]
QacR variant PqacA Escherichia coli [74]
nd PyeiW Escherichia coli [64]
EmrR Engineered PemrR Escherichia coli [67]
EmrR PemrR Escherichia coli [79]
YqhC PyqhD Escherichia coli [102]
PcaV variant Engineered PPV Escherichia coli [76]
VanR Engineered PVanO Escherichia coli [103]
nd PLPD00563 Rhodococcus opacus [104]
 Vanillic acid VanR PvanCC Escherichia coli [101]
EmrR PemrR Escherichia coli [67]
EmrR PemrR Escherichia coli [79]
 p-Coumaric acid EmrR Engineered PemrR Escherichia coli [67]
EmrR PemrR Escherichia coli [79]
PadR PpadC Corynebacterium glutamicum [48]
 Ferulic acid and related compounds FerC Engineered PLC and PPC Escherichia coli [66]
FerC Pech Escherichia coli [88]
 Cinnamaldehyde BldR PSso2536adh Escherichia coli [105]
 Benzoic acid EmrR PemrR Escherichia coli [79]
NahR Psal Escherichia coli [85]
BenR PbenA Escherichia coli [80]
nd PLPD06580 Rhodococcus opacus [104]
 4-Hydroxybenzoic acid EmrR PemrR Escherichia coli [79]
PobR variant Ppob Escherichia coli, Pseudomonas putida [75, 86]
nd PLPD06764 Rhodococcus opacus [104]
 2-Hydroxybenzoic acid NahR Psal Escherichia coli [85]
 Benzaldehyde BldR PSso2536adh Escherichia coli [105]
 Benzoic acid derivatives XylS Pm Escherichia coli [71]
 Salicylic acid EmrR PemrR Escherichia coli [79]
Engineered AraC PBAD Escherichia coli [106]
 Salicylaldehyde BldR PSso2536adh Escherichia coli [105]
NahR Psal Escherichia coli [85]
 Salicylic acid derivatives XylS, NahR Pm, Psal Escherichia coli [71]
 Phenol EmrR PemrR Escherichia coli [79]
 Phenol derivatives DmpR Po Escherichia coli [71]
 Syringaldehyde EmrR PemrR Escherichia coli [68]
 BTX XylR Engineered Po’ Escherichia coli [65]
 Other aromatic aldehydes BldR PSso2536adh Escherichia coli [105]
PcaV variant Engineered PPV Escherichia coli [76]
C5/C6 sugars
 Xylose XBP−XynA PxylF Escherichia coli [107]
XylR Engineered PGAL1/XylR Saccharomyces cerevisiae [91]
XylR–XBP PxylF Escherichia coli [95]
 d-Arabinose araF FRET Escherichia coli [94]
AraC variant PBAD Escherichia coli [90]
 l-Rhamnose RhaS PrhaBAD Escherichia coli [96]
 l-Mannose RhaS PrhaBAD Escherichia coli [96]
 Cellulase CelR PTRC Escherichia coli [92]
 Maltose AraF FRET Escherichia coli [94]
 Glucose, glutamate MglB, YbeJ FRET Escherichia coli [93]
  1. nd not determined