Table 1 Genetically encoded biosensors developed and applied to sense aromatic monomers and sugars towards lignin valorization
From: Genetically encoded biosensors for lignocellulose valorization
Target molecule(s) | Sensing element | Output element | Host organism | References |
|---|---|---|---|---|
Aromatic monomers | ||||
Protocatechuic acid | PcaU | Engineered PpcaU, P3B5B | Escherichia coli | |
PcaU | Engineered PpcaU | Pseudomonas putida | [70] | |
PobR variant | Ppob | Escherichia coli | [75] | |
PcaV | Engineered PPV | Escherichia coli | [76] | |
Vanillin | EmrR | PemrR | Escherichia coli | [68] |
Yap1/Msn2 | Padh7 | Escherichia coli | [89] | |
QacR variant | PqacA | Escherichia coli | [74] | |
nd | PyeiW | Escherichia coli | [64] | |
EmrR | Engineered PemrR | Escherichia coli | [67] | |
EmrR | PemrR | Escherichia coli | [79] | |
YqhC | PyqhD | Escherichia coli | [102] | |
PcaV variant | Engineered PPV | Escherichia coli | [76] | |
VanR | Engineered PVanO | Escherichia coli | [103] | |
nd | PLPD00563 | Rhodococcus opacus | [104] | |
Vanillic acid | VanR | PvanCC | Escherichia coli | [101] |
EmrR | PemrR | Escherichia coli | [67] | |
EmrR | PemrR | Escherichia coli | [79] | |
p-Coumaric acid | EmrR | Engineered PemrR | Escherichia coli | [67] |
EmrR | PemrR | Escherichia coli | [79] | |
PadR | PpadC | Corynebacterium glutamicum | [48] | |
Ferulic acid and related compounds | FerC | Engineered PLC and PPC | Escherichia coli | [66] |
FerC | Pech | Escherichia coli | [88] | |
Cinnamaldehyde | BldR | PSso2536adh | Escherichia coli | [105] |
Benzoic acid | EmrR | PemrR | Escherichia coli | [79] |
NahR | Psal | Escherichia coli | [85] | |
BenR | PbenA | Escherichia coli | [80] | |
nd | PLPD06580 | Rhodococcus opacus | [104] | |
4-Hydroxybenzoic acid | EmrR | PemrR | Escherichia coli | [79] |
PobR variant | Ppob | Escherichia coli, Pseudomonas putida | ||
nd | PLPD06764 | Rhodococcus opacus | [104] | |
2-Hydroxybenzoic acid | NahR | Psal | Escherichia coli | [85] |
Benzaldehyde | BldR | PSso2536adh | Escherichia coli | [105] |
Benzoic acid derivatives | XylS | Pm | Escherichia coli | [71] |
Salicylic acid | EmrR | PemrR | Escherichia coli | [79] |
Engineered AraC | PBAD | Escherichia coli | [106] | |
Salicylaldehyde | BldR | PSso2536adh | Escherichia coli | [105] |
NahR | Psal | Escherichia coli | [85] | |
Salicylic acid derivatives | XylS, NahR | Pm, Psal | Escherichia coli | [71] |
Phenol | EmrR | PemrR | Escherichia coli | [79] |
Phenol derivatives | DmpR | Po | Escherichia coli | [71] |
Syringaldehyde | EmrR | PemrR | Escherichia coli | [68] |
BTX | XylR | Engineered Po’ | Escherichia coli | [65] |
Other aromatic aldehydes | BldR | PSso2536adh | Escherichia coli | [105] |
PcaV variant | Engineered PPV | Escherichia coli | [76] | |
C5/C6 sugars | ||||
Xylose | XBP−XynA | PxylF | Escherichia coli | [107] |
XylR | Engineered PGAL1/XylR | Saccharomyces cerevisiae | [91] | |
XylR–XBP | PxylF | Escherichia coli | [95] | |
d-Arabinose | araF | FRET | Escherichia coli | [94] |
AraC variant | PBAD | Escherichia coli | [90] | |
l-Rhamnose | RhaS | PrhaBAD | Escherichia coli | [96] |
l-Mannose | RhaS | PrhaBAD | Escherichia coli | [96] |
Cellulase | CelR | PTRC | Escherichia coli | [92] |
Maltose | AraF | FRET | Escherichia coli | [94] |
Glucose, glutamate | MglB, YbeJ | FRET | Escherichia coli | [93] |