Fig. 4
Growth and photosynthesis rates of the wild type and mutants 283 and 359 of Chlorella vulgaris ESP-31 in a closed photobioreactor aerated with simulated flue gas under outdoor cultivation. a Growth curves, b Nitrate concentration, c net photosynthesis rate, d Nile red staining. Bright field: A, C, E, G, I, K. Fluorescence: B, D, F, H, J, L. A 1800-mL culture of each strain aerated with simulated flue gas (0.1 vvm) was grown in ¼ N BG-11 medium (containing 0.375 g/L NaNO3) at daytime and then aerated with air (0.1 vvm) at night for 6 days. Next, cells were cultivated by aeration with air (0.1 vvm) until 30 days. Data shown are the means (along with the standard deviations) of at least two independent experiments