Skip to main content

Table 1 Operating parameter and conditions

From: Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production

No. Operating parameter Initial condition Variables Unit
1 Concentration of hydrogen peroxide 5 5, 10, 15, 20, 25, 30 %w/w
2 Type of solvents and concentration Hexane, 20 Hexane: 20, 40, 60, 80, ~ 85 (Max)
Chloroform: 20, 40, 60, 80, ~ 99.5 (Max)
%w/w
3 Contact time 1 min 1, 2, 3, 4 min
4 Biomass amounts 20 20, 30, 40, 50 mg
5 Ratio of solvents—cell disruption solvent (hydrogen peroxide): organic solvent (hexane) 1:1 1:1, 1:2, 1:3, 2:1, 2:3, 3:1, 3:2
6 Volume—hydrogen peroxide (X), organic solvent (Y) X: 1, Y: 3 Hydrogen peroxide (X): 1, 2, 3, 4
Organic solvent (Y): 3, 4, 5, 6
mL