Table 1 Operating parameter and conditions

1

Concentration of hydrogen peroxide

5

5, 10, 15, 20, 25, 30

%w/w

2

Type of solvents and concentration

Hexane, 20

Hexane: 20, 40, 60, 80, ~ 85 (Max)

Chloroform: 20, 40, 60, 80, ~ 99.5 (Max)

%w/w

3

Contact time

1 min

1, 2, 3, 4

min

4

Biomass amounts

20

20, 30, 40, 50

mg

5

Ratio of solvents—cell disruption solvent (hydrogen peroxide): organic solvent (hexane)

1:1

1:1, 1:2, 1:3, 2:1, 2:3, 3:1, 3:2

–

6

Volume—hydrogen peroxide (X), organic solvent (Y)

X: 1, Y: 3

Hydrogen peroxide (X): 1, 2, 3, 4

Organic solvent (Y): 3, 4, 5, 6

mL