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Table 2 Production of DHA by various marine thraustochytrids

From: Simultaneous production of DHA and squalene from Aurantiochytrium sp. grown on forest biomass hydrolysates

Microorganisms Cultivation mode Glucose Nitrogen Cell dry weight (g/L) Lipid concentration (g/L) DHA concentration (%, total lipid) References
Aurantiochytrium sp. ATCC PRA-276 Batch in flask Glucose (30 g/L) 3.0 g/L (1.36 g/L (NH4)2SO4, 13.63 g/L yeast extract and 13.63 g/L monosodium glutamate) 9.3 5.5 [44]
0.44 g/L (0.1 g/L de (NH4)2SO4, 1.0 g/L yeast extract, 1.0 g/L monosodium glutamate) 17.0 12.5
Aurantiochytrium sp. KRS101 Baffled flask Orange peel extract glucose (5.9 g/L), fructose (5.6 g/L), organic acids NaNO3 (1.2 g/L) 4.40 2.12 (FAME) 14.31 [62]
5 g/L glucose + orange peel extract glucose (5.9 g/L), fructose (5.6 g/L), organic acids NaNO3 (1.5 g/L) 5.50 2.85 14.18
Aurantiochytrium sp. KRS101 Flask Modified basal medium glucose (60 g/L) Corn steep solid 9.00 3.42 19.88 [34]
Schizochytrium limacinum SR 21 Flask Glucose (90 g/L) Yeast extract (2 g/L) 18.2 24.2 14.72 [63]
Glycerol (100 g/L) Yeast extract (2 g/L) 20.10 15.03 18.38
Aurantiochytrium 4 W-1b Flask Glucose (30 g/L) 1% tryptone and 0.5% yeast extract 9.01 ± 0.62 4.16 ± 0.48 27.9 [35]
Aurantiochytrium SW1 Flask Fructose (70 g/L) 10 g/L monosodium glutamate 19.0 9.13 25 [39]
Aurantiochytrium sp. T66 (ATCC PRA-276) Flask Organosolv-pretreated birch hydrolysate (30 g/L glucose) Yeast extract (C:N, 10) 10.39 ± 0.73 4.98 ± 0.38 25.98 ± 1.20 This study
Bioreactor 11.24 ± 0.79 5.90 ± 0.43 35.76 ± 0.98