Saccharomyces cerevisiae exhibiting a modified route for uptake and catabolism of glycerol forms significant amounts of ethanol from this carbon source considered as ‘non-fermentable’

Table 1 List of all S. cerevisiae strains and all plasmids used in this study

Strain	Genome modifications	References
CBS 6412-13A	–	Swinnen et al. [18]
CBS 6412-13A FPS1	YGLCτ3:: PGK1p-CjFPS1-RPL15At:loxP-ble-loxP	This study
CBS DHA	YPRCΔ15::ACT1p-DAK1-TPS1t; YPRCτ3::TEF1p-Opgdh-CYC1t; gut1::loxP	Aßkamp et al. [27]
CBS DHA FPS1	YPRCΔ15::ACT1p-DAK1-TPS1t; YPRCτ3::TEF1p-Opgdh-CYC1t; gut1::loxP; YGLCτ3::PGK1p-CjFPS1-RPL15At:loxP-ble-loxP	This study

Plasmid	Relevant characteristics	References
p426-SNR52p-gRNA-YGLCτ3-SUP4t-hphMX	gRNA targeting YGLCτ3; hygromycin B resistance	Klein et al. [24]
pUC18-PGK1p-CjFPS1	CjFPS1 under control of PGK1p and RPL15At	Islam et al. [26]
p414-TEF1p-Cas9-CYC1t-nat1	Cas9 from S. pyogenes; nourseothricin resistance	Klein et al. [24]

ISSN: 2731-3654