Table 3 Comparison of enzyme activity and production between wild-type D2-BGL and the N68Q, T431A and T431S mutants
Activity (U/mL) | Specific activity (U/mg) | Productivity (mg/L) | |
|---|---|---|---|
Wild type | 1.89 ± 0.18 | 197 ± 3 | 9.6 |
N68Q | 0.98 ± 0.22 | 216 ± 23 | 4.5 |
T431A | 0.61 ± 0.05 | 189 ± 11 | 3.2 |
T431S | 1.89 ± 0.03 | 225 ± 20 | 8.4 |