Fig. 1

Sequence properties of alginate lyases AlyPB1 and AlyPB2 from Photobacterium sp. FC615. a Module organization of the alginate lyases AlyPB1 and AlyPB2. AlyPB1 contains a putative N-terminal signal peptide (Met¹-Ala²¹) and a chondroitinase B domain (Thr⁷⁴-Asp⁴⁷⁰). AlyPB2 contains an unknown function module DUF4962 (Phe²⁹-Gln³²⁰) at N-terminal and a heparinase II/III-like superfamily module (Pro³⁹⁶-His⁵⁷¹) at C-terminal. b Phylogenetic analysis of alginate lyases AlyPB1 and AlyPB2 based on protein sequence alignments with reported alginate lyases in various polysaccharide lyase families. The phylogenetic tree was constructed using MEGA version 5.05 software via the neighbor-joining algorithm and associated taxa clustered together in the bootstrap test of 1000 replicates