Fig. 2
Purification of recombinant AlyPB1 (a) and AlyPB2 (b) from E. coli by Ni2+ chelation chromatography. Enzyme purity following each fractionation step was assessed by SDS-PAGE using 13.2% (w/v) polyacrylamide gels, followed by staining with Coomassie Brilliant Blue. Lane 1, unstained protein molecular weight marker ProteinRuler® II (TransGen Biotech); Lane 2, induced cell lysate of E. coli cells harboring the control plasmid pET-22b (+); Lane 3, induced cell lysate of E. coli cells containing plasmid of pET22b-alyPB1 (a) or pET22b-alyPB2 (b); Lane 4, supernatant fluid of the induced cell lysate; Lane 5, purified recombinant AlyPB1 (a) or AlyPB2 (b)