Fig. 8

Analysis for synergistic effect of AlyPB1 and AlyPB2. a The enzyme activities of exo-lyase AlyPB2 were determined using the alginate with different molecular sizes as substrates. Unsaturated oligosaccharide substrates UDP2–UDP10 were prepared from the partial digestion of alginate by endo-lyase AlyPB1. 10 kDa and 25 kDa alginate polysaccharide were prepared from acid hydrolysis of alginate. Data are shown as the percentage of the activity on alginate (100%). b Alginate polysaccharide was treated with a mixture (100 mU) of endo-lyase AlyPB1 and exo-lyase AlyPB2 with an activity ratio of 1:1 at 20 °C for 10 min. By contrast, control was treated with only 100 mU of AlyPB1 or AlyPB2 at the same reaction conditions. The resulting products were analysed by gel filtration as described above at a wavelength of 235 nm. UDP1, UDP2, UDP3, and UDP4 represent unsaturated alginate oligosaccharides as mentioned above