Strains and plasmids | Description | Source |
---|---|---|
Strains | ||
 Photobacterium sp. FC615 | A GAG-degrading marine bacterium | This study |
 E. coli BL21(DE3) | F-, ompT, hsdSB (rB-, mB-), dcm, gal, λ (DE3), pLysS, Cmr | Novagen |
Plasmids | ||
 pET-22b | Expression vector; Apr | Novagen |
 pET22b-alyPB1 | pET-22b carrying an amplified NdeI-XhoI fragment encoding the recombinant protein of AlyPB1 or AlyPB2 fused with a His6 tag at the C terminus | This study |
 pET22b-alyPB2 | ||
Sequencing primers | ||
 AlyPB1-F | 5′-CATATGTCGACCCAAGATACACCAGTACCGG-3′ |  |
 AlyPB1-R | 5′-CTCGAGGCTCTTCGGTGCAACCTGCAAACG-3′ |  |
 AlyPB2-F | 5′-CATATGAAGCTGGAGAATGATACTTCAGCA-3′ |  |
 AlyPB2-R | 5′-CTCGAGCAGCTCGATAGTCACTAACTCGCC-3′ |  |