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Table 1 Bacterial strains, plasmids, and primers used for sequencing in the present study

From: Biochemical characteristics and synergistic effect of two novel alginate lyases from Photobacterium sp. FC615

Strains and plasmids

Description

Source

Strains

 Photobacterium sp. FC615

A GAG-degrading marine bacterium

This study

 E. coli BL21(DE3)

F-, ompT, hsdSB (rB-, mB-), dcm, gal, λ (DE3), pLysS, Cmr

Novagen

Plasmids

 pET-22b

Expression vector; Apr

Novagen

 pET22b-alyPB1

pET-22b carrying an amplified NdeI-XhoI fragment encoding the recombinant protein of AlyPB1 or AlyPB2 fused with a His6 tag at the C terminus

This study

 pET22b-alyPB2

Sequencing primers

 AlyPB1-F

5′-CATATGTCGACCCAAGATACACCAGTACCGG-3′

 

 AlyPB1-R

5′-CTCGAGGCTCTTCGGTGCAACCTGCAAACG-3′

 

 AlyPB2-F

5′-CATATGAAGCTGGAGAATGATACTTCAGCA-3′

 

 AlyPB2-R

5′-CTCGAGCAGCTCGATAGTCACTAACTCGCC-3′

 
  1. Restriction enzyme sites are underlined
  2. Apr ampicillin-resistant