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Table 1 Bacterial strains, plasmids, and primers used for sequencing in the present study

From: Biochemical characteristics and synergistic effect of two novel alginate lyases from Photobacterium sp. FC615

Strains and plasmids Description Source
Strains
 Photobacterium sp. FC615 A GAG-degrading marine bacterium This study
 E. coli BL21(DE3) F-, ompT, hsdSB (rB-, mB-), dcm, gal, λ (DE3), pLysS, Cmr Novagen
Plasmids
 pET-22b Expression vector; Apr Novagen
 pET22b-alyPB1 pET-22b carrying an amplified NdeI-XhoI fragment encoding the recombinant protein of AlyPB1 or AlyPB2 fused with a His6 tag at the C terminus This study
 pET22b-alyPB2
Sequencing primers
 AlyPB1-F 5′-CATATGTCGACCCAAGATACACCAGTACCGG-3′  
 AlyPB1-R 5′-CTCGAGGCTCTTCGGTGCAACCTGCAAACG-3′  
 AlyPB2-F 5′-CATATGAAGCTGGAGAATGATACTTCAGCA-3′  
 AlyPB2-R 5′-CTCGAGCAGCTCGATAGTCACTAACTCGCC-3′  
  1. Restriction enzyme sites are underlined
  2. Apr ampicillin-resistant
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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