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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Metabolic engineering Escherichia coli for efficient production of icariside D2

Fig. 5

The E. coliE. coli coculture for production of icariside D2 using glucose–xylose mixture. a The E. coliE. coli coculture strategy for production of icariside D2. Strain BMT23 produced tyrosol preferentially using xylose, while strain BMD12 synthesized icariside D2 using glucose. X5P xylulose 5-phosphate, F6P fructose 6-phosphate, G3P glyceraldehyde 3-phosphate. b Effect of the ushA gene on icariside D2 biosynthesis. The ushA gene was deleted in strain BMD12 and not in strain BMD2. Fermentation was done with supplementation of 500 mg/L tyrosol. c Optimization of icariside D2 production by varying the inoculation ratio of BMD12 to BMT23 with the G/X ratio of 1/1. d Optimization of icariside D2 production by varying the ratio of glucose to xylose with the initial T/D ratio of 2/1

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