Table 2 Bacterial strains and plasmids used in this study
From: Metabolic engineering Escherichia coli for efficient production of icariside D2
Strain | Characteristics | Source |
|---|---|---|
E. coli BW25113 | lacIqrrnBT14ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | NBRP-E. coli at NIG |
BAK5 | BW25113∆ptsG::∆tyrR::∆pykA:: pykF::∆pheA::FRT | [29] |
BAK10 | BAK5Δmao-paa cluster::PlacUV5-aroGfbr-tyrAfbr-aroE | [30] |
BMT18 | BAK10 ΔfeaB::FRT | [13] |
BMT18(DE3) | BMT18 PlacUV5-T7 RNA pol | This study |
BMT23 | BAK10 ΔfeaB::FRT ΔmanZ::chl pLX2 | [13] |
BMD1 | BL21(DE3) pLXD1 | This study |
BMD2 | BL21(DE3) pLXD2 | This study |
BMD3 | BL21(DE3) pLXD3 | This study |
BMD4 | BL21(DE3) pLXD4 | This study |
BMD5 | BL21(DE3) pLXD5 | This study |
BMD6 | BL21(DE3) pLXD6 | This study |
BMD7 | BL21(DE3) pLXD7 | This study |
BMD8 | BL21(DE3) pLXD8 | This study |
BMD9 | BL21(DE3) pLX2 pLXD8 | This study |
BMD10 | BMT18(DE3) pLXD9 | This study |
BMD11 | BL21(DE3) ΔushA::kan ΔxylA::FRT | This study |
BMD12 | BMD11 pLXD2 | This study |