Table 3 Plasmids used in this study
From: Metabolic engineering Escherichia coli for efficient production of icariside D2
Plasmid | Characteristics | Source |
|---|---|---|
pKD46 | Red recombinase expression vector; AmpR | [45] |
pKD3 | FRT (FLP recognition target) sites; CmR | [45] |
pKD4 | FRT (FLP recognition target) sites; KanR | [45] |
pCP20 | FLP expression vector; AmpR | [45] |
pCDFDuet-1 | pCDF ori with PT7; SmR | Novagen |
PGEX-6P-1 | colE1 ori with Ptac; AmpR | Novagen |
pTrc99A | colE1 ori with Ptrc; AmpR | Novagen |
pETDuet-1 | colE1 ori with PT7; AmpR | Novagen |
pACYCDuet-1 | p15A ori with PT7; CmR | Novagen |
pRSFDuet-1 | RSF ori with PT7; KanR | Novagen |
pLXD1 | pCDFDuet-1 with RcUGT1 | This study |
pLXD2 | pCDFDuet-1 with RrUGT3 | This study |
pLXD3 | pCDFDuet-1 with RsUGT72B14 | This study |
pLXD 4 | pTrc99A with RrUGT3 | This study |
pLXD 5 | PGEX-6P-1 with RrUGT3 | This study |
pLXD 6 | pETDuet-1 with RrUGT3 | This study |
pLXD 7 | pACYCDuet-1 with RrUGT3 | This study |
pLXD 8 | pRSFDuet-1 with RrUGT3 | This study |
pLX2 | pTrc99a (with deletion of lacI sequence and change from ampR to strR) with synkdc4 | [13] |
pLXD9 | pLX2 with T7-RrUGT3 | This study |