Skip to main content


Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Integrated strain- and process design enable production of 220 g L−1 itaconic acid with Ustilago maydis

Fig. 4

Controlled pulsed fed-batch fermentation of U. maydis MB215 ∆cyp3 ∆Pria1::Peteffuz7 PetefmttA in the presence of CaCO3. a Growth (filled triangle), and glucose concentration (filled square) and b: concentration of itaconate (filled inverted triangle), malate (open inverted triangle), and pH (continuous line) during a single representative bioreactor cultivation in batch medium with a starting concentration of 50 g L−1 glucose and 1.6 g L−1 NH4Cl. pH was kept above 6.2 by manual addition of CaCO3. Feeding schemes of glucose and CaCO3 are listed in Additional file 1: Table S1

Back to article page