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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Integrated strain- and process design enable production of 220 g L−1 itaconic acid with Ustilago maydis

Fig. 5

Controlled high-density pulsed fed-batch fermentation of U. maydis MB215 ∆cyp3 ∆Pria1::Peteffuz7 PetefmttA in the presence of CaCO3. a Growth (filled triangle), and glucose concentration (filled square) and b concentration of itaconate (filled inverted triangle), malate (open inverted triangle), and pH (continuous line) during a single representative bioreactor cultivation in batch medium with a starting concentration of 200 g L−1 glucose and 4 g L−1 NH4Cl. The pH was kept above 6.2 by manual addition of CaCO3. Feeding schemes of glucose and CaCO3 are listed in Additional file 1: Table S2

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